Detailed information of TA system TA098366

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4312773..4313571
• Replicon: chromosome
• Accession: NZ_CP027366
• Organism: Escherichia coli strain 89-3156

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: C6P65_RS22495
• Protein ID: WP_000854903.1
• Coordinates: 4313194..4313571 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A093DMQ4
• Locus tag: C6P65_RS22490
• Protein ID: WP_001280952.1
• Coordinates: 4312773..4313147 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P65_RS22465	4310153..4310386	+	234	WP_001117559.1	DUF905 family protein	-
C6P65_RS22470	4310476..4311294	+	819	WP_073569129.1	DUF945 domain-containing protein	-
C6P65_RS22475	4311349..4311834	+	486	WP_073569128.1	antirestriction protein	-
C6P65_RS22480	4311850..4312326	+	477	WP_001186726.1	RadC family protein	-
C6P65_RS22485	4312389..4312610	+	222	WP_000692311.1	DUF987 domain-containing protein	-
C6P65_RS22490	4312773..4313147	+	375	WP_001280952.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6P65_RS22495	4313194..4313571	+	378	WP_000854903.1	TA system toxin CbtA family protein	Toxin
C6P65_RS22500	4313568..4314056	+	489	WP_001562324.1	hypothetical protein	-
C6P65_RS22505	4314068..4314265	+	198	WP_001562326.1	DUF957 domain-containing protein	-
C6P65_RS22510	4314350..4315195	+	846	WP_086258116.1	DUF4942 domain-containing protein	-
C6P65_RS22525	4315988..4317172	+	1185	WP_001172876.1	sugar efflux transporter	-
C6P65_RS22530	4317283..4318206	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-118)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14338.30 Da
• Isoelectric Point: 6.8528

>T98366 WP_000854903.1 NZ_CP027366:4313194-4313571 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATDLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T98366 NZ_CP027366:4313194-4313571 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGACCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.50 Da
• Isoelectric Point: 6.6249

>AT98366 WP_001280952.1 NZ_CP027366:4312773-4313147 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Nucleotide

• Length: 375 bp

>AT98366 NZ_CP027366:4312773-4313147 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A093DMQ4