Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3311058..3311712 Replicon chromosome
Accession NZ_CP027366
Organism Escherichia coli strain 89-3156

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag C6P65_RS17175 Protein ID WP_000244777.1
Coordinates 3311058..3311465 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6P65_RS17180 Protein ID WP_000354046.1
Coordinates 3311446..3311712 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P65_RS17155 3307015..3308748 - 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -
C6P65_RS17160 3308754..3309464 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6P65_RS17165 3309489..3310385 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6P65_RS17170 3310497..3311018 + 522 WP_001055874.1 flavodoxin FldB -
C6P65_RS17175 3311058..3311465 - 408 WP_000244777.1 toxin CptA Toxin
C6P65_RS17180 3311446..3311712 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6P65_RS17185 3311955..3312935 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
C6P65_RS17190 3313131..3313790 - 660 WP_000250274.1 hemolysin III family protein -
C6P65_RS17195 3313954..3314265 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6P65_RS17200 3314310..3315743 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
C6P65_RS17205 3315800..3316543 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T98363 WP_000244777.1 NZ_CP027366:c3311465-3311058 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98363 NZ_CP027366:c3311465-3311058 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98363 WP_000354046.1 NZ_CP027366:c3311712-3311446 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98363 NZ_CP027366:c3311712-3311446 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References