Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4365988..4366606 Replicon chromosome
Accession NZ_CP027362
Organism Escherichia coli strain 95-3192

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6P51_RS22865 Protein ID WP_001291435.1
Coordinates 4365988..4366206 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6P51_RS22870 Protein ID WP_000344800.1
Coordinates 4366232..4366606 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P51_RS22830 4361277..4361849 + 573 WP_000779831.1 YbaY family lipoprotein -
C6P51_RS22835 4361880..4362191 - 312 WP_000409911.1 MGMT family protein -
C6P51_RS22845 4362570..4362923 + 354 WP_000878140.1 DUF1428 domain-containing protein -
C6P51_RS22850 4362965..4364515 - 1551 WP_024221674.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6P51_RS22855 4364679..4365149 - 471 WP_000136192.1 YlaC family protein -
C6P51_RS22860 4365265..4365816 - 552 WP_000102564.1 maltose O-acetyltransferase -
C6P51_RS22865 4365988..4366206 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6P51_RS22870 4366232..4366606 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6P51_RS22875 4367152..4370301 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C6P51_RS22880 4370324..4371517 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98337 WP_001291435.1 NZ_CP027362:c4366206-4365988 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98337 NZ_CP027362:c4366206-4365988 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98337 WP_000344800.1 NZ_CP027362:c4366606-4366232 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98337 NZ_CP027362:c4366606-4366232 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References