Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3937245..3937795 Replicon chromosome
Accession NZ_CP027362
Organism Escherichia coli strain 95-3192

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag C6P51_RS20770 Protein ID WP_001160974.1
Coordinates 3937481..3937795 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag C6P51_RS20765 Protein ID WP_000125566.1
Coordinates 3937245..3937478 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P51_RS20740 3932316..3932603 + 288 WP_000203748.1 ferredoxin-like protein FixX -
C6P51_RS20745 3932661..3933992 + 1332 WP_001183192.1 MFS transporter -
C6P51_RS20750 3934103..3934633 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
C6P51_RS20755 3934626..3936488 + 1863 WP_000377137.1 glutathione-regulated potassium-efflux system protein KefC -
C6P51_RS20760 3936680..3937159 + 480 WP_024221573.1 type 3 dihydrofolate reductase -
C6P51_RS20765 3937245..3937478 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
C6P51_RS20770 3937481..3937795 + 315 WP_001160974.1 CcdB family protein Toxin
C6P51_RS20775 3937792..3938640 - 849 WP_000257203.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
C6P51_RS20780 3938647..3939024 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
C6P51_RS20785 3939027..3939848 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
C6P51_RS20790 3939845..3940834 - 990 WP_000241240.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
C6P51_RS20795 3940834..3942120 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T98336 WP_001160974.1 NZ_CP027362:3937481-3937795 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T98336 NZ_CP027362:3937481-3937795 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT98336 WP_000125566.1 NZ_CP027362:3937245-3937478 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT98336 NZ_CP027362:3937245-3937478 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References