Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2675527..2676158 Replicon chromosome
Accession NZ_CP027362
Organism Escherichia coli strain 95-3192

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag C6P51_RS14495 Protein ID WP_001259387.1
Coordinates 2675883..2676158 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag C6P51_RS14490 Protein ID WP_000593555.1
Coordinates 2675527..2675886 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P51_RS14460 2670639..2671112 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
C6P51_RS14465 2671109..2671390 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
C6P51_RS14470 2671467..2672825 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
C6P51_RS14475 2672818..2674326 + 1509 WP_001400448.1 carbohydrate kinase -
C6P51_RS14480 2674316..2674585 + 270 WP_000933061.1 HPr family phosphocarrier protein -
C6P51_RS14485 2674617..2675477 + 861 WP_001131788.1 class II aldolase -
C6P51_RS14490 2675527..2675886 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
C6P51_RS14495 2675883..2676158 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
C6P51_RS14500 2676231..2677355 - 1125 WP_001314210.1 ABC transporter permease -
C6P51_RS14505 2677355..2680090 - 2736 WP_000149086.1 ribosome-associated ATPase/putative transporter RbbA -
C6P51_RS14510 2680087..2681154 - 1068 WP_000361517.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T98333 WP_001259387.1 NZ_CP027362:c2676158-2675883 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T98333 NZ_CP027362:c2676158-2675883 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACAGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT98333 WP_000593555.1 NZ_CP027362:c2675886-2675527 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT98333 NZ_CP027362:c2675886-2675527 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAGCTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References