Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 4415698..4416169 Replicon chromosome
Accession NZ_CP027361
Organism Escherichia coli strain 2014C-4639

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag RR62_RS22510 Protein ID WP_001448501.1
Coordinates 4415891..4416169 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag RR62_RS22505 Protein ID WP_001302048.1
Coordinates 4415698..4415889 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR62_RS22455 (4410776) 4410776..4410994 - 219 WP_000683609.1 DUF4014 family protein -
RR62_RS22460 (4411122) 4411122..4411433 - 312 WP_001289673.1 hypothetical protein -
RR62_RS22465 (4411426) 4411426..4411653 - 228 WP_000699809.1 hypothetical protein -
RR62_RS22470 (4411650) 4411650..4411931 - 282 WP_000603384.1 DNA-binding protein -
RR62_RS22475 (4411964) 4411964..4412725 - 762 WP_000450872.1 DUF1627 domain-containing protein -
RR62_RS22480 (4412751) 4412751..4413492 - 742 Protein_4362 ATP-binding protein -
RR62_RS22485 (4413499) 4413499..4414461 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
RR62_RS22490 (4414484) 4414484..4414909 - 426 WP_000693943.1 toxin YdaT family protein -
RR62_RS22495 (4414906) 4414906..4415208 - 303 WP_000172738.1 transcriptional regulator -
RR62_RS22500 (4415306) 4415306..4415677 + 372 WP_001169687.1 hypothetical protein -
RR62_RS22505 (4415698) 4415698..4415889 + 192 WP_001302048.1 hypothetical protein Antitoxin
RR62_RS22510 (4415891) 4415891..4416169 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RR62_RS22515 (4416456) 4416456..4416608 + 153 WP_001427316.1 DUF1391 family protein -
RR62_RS22520 (4417029) 4417029..4417250 + 222 WP_000560218.1 killing protein KilR -
RR62_RS22525 (4417250) 4417250..4417420 + 171 WP_001427414.1 YdaE family protein -
RR62_RS22530 (4417494) 4417494..4417769 + 276 WP_001307773.1 hypothetical protein -
RR62_RS22535 (4417868) 4417868..4420519 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleC 4371193..4429046 57853


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T98308 WP_001448501.1 NZ_CP027361:4415891-4416169 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98308 NZ_CP027361:4415891-4416169 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98308 WP_001302048.1 NZ_CP027361:4415698-4415889 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98308 NZ_CP027361:4415698-4415889 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References