Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4097768..4098406 Replicon chromosome
Accession NZ_CP027361
Organism Escherichia coli strain 2014C-4639

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag RR62_RS20720 Protein ID WP_000813794.1
Coordinates 4097768..4097944 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag RR62_RS20725 Protein ID WP_001270286.1
Coordinates 4097990..4098406 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR62_RS20700 (4093387) 4093387..4094601 - 1215 WP_001307190.1 BenE family transporter YdcO -
RR62_RS20705 (4094654) 4094654..4095190 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
RR62_RS20710 (4095263) 4095263..4097224 + 1962 WP_001341357.1 23S rRNA 5-hydroxycytidine C2501 synthase -
RR62_RS20715 (4097316) 4097316..4097546 - 231 WP_000494244.1 YncJ family protein -
RR62_RS20720 (4097768) 4097768..4097944 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
RR62_RS20725 (4097990) 4097990..4098406 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
RR62_RS20730 (4098485) 4098485..4099890 + 1406 Protein_4010 PLP-dependent aminotransferase family protein -
RR62_RS20735 (4100135) 4100135..4101280 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
RR62_RS20740 (4101298) 4101298..4102311 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
RR62_RS20745 (4102312) 4102312..4103253 + 942 WP_001251332.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4092199..4093347 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T98305 WP_000813794.1 NZ_CP027361:4097768-4097944 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T98305 NZ_CP027361:4097768-4097944 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT98305 WP_001270286.1 NZ_CP027361:4097990-4098406 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT98305 NZ_CP027361:4097990-4098406 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References