Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2727798..2728416 Replicon chromosome
Accession NZ_CP027361
Organism Escherichia coli strain 2014C-4639

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag RR62_RS13405 Protein ID WP_001291435.1
Coordinates 2727798..2728016 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag RR62_RS13410 Protein ID WP_000344800.1
Coordinates 2728042..2728416 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR62_RS13370 (2723088) 2723088..2723660 + 573 WP_000779826.1 YbaY family lipoprotein -
RR62_RS13375 (2723691) 2723691..2724002 - 312 WP_000409911.1 MGMT family protein -
RR62_RS13385 (2724380) 2724380..2724733 + 354 WP_000878140.1 DUF1428 domain-containing protein -
RR62_RS13390 (2724775) 2724775..2726325 - 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
RR62_RS13395 (2726489) 2726489..2726959 - 471 WP_000136192.1 YlaC family protein -
RR62_RS13400 (2727075) 2727075..2727626 - 552 WP_000102564.1 maltose O-acetyltransferase -
RR62_RS13405 (2727798) 2727798..2728016 - 219 WP_001291435.1 HHA domain-containing protein Toxin
RR62_RS13410 (2728042) 2728042..2728416 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
RR62_RS13415 (2728962) 2728962..2732111 - 3150 WP_001132475.1 efflux RND transporter permease AcrB -
RR62_RS13420 (2732134) 2732134..2733327 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98296 WP_001291435.1 NZ_CP027361:c2728016-2727798 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98296 NZ_CP027361:c2728016-2727798 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98296 WP_000344800.1 NZ_CP027361:c2728416-2728042 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98296 NZ_CP027361:c2728416-2728042 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References