Detailed information of TA system TA098295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 2693501..2694338
• Replicon: chromosome
• Accession: NZ_CP027361
• Organism: Escherichia coli strain 2014C-4639

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: RR62_RS13235
• Protein ID: WP_000227784.1
• Coordinates: 2693501..2694043 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: RR62_RS13240
• Protein ID: WP_001297137.1
• Coordinates: 2694027..2694338 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR62_RS13215 (2689040)	2689040..2689951	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
RR62_RS13220 (2690119)	2690119..2690610	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
RR62_RS13225 (2690738)	2690738..2692102	-	1365	WP_001000977.1	MFS transporter	-
RR62_RS13230 (2692510)	2692510..2693445	+	936	WP_001375746.1	tetratricopeptide repeat protein	-
RR62_RS13235 (2693501)	2693501..2694043	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
RR62_RS13240 (2694027)	2694027..2694338	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
RR62_RS13245 (2694523)	2694523..2695413	-	891	WP_000971336.1	heme o synthase	-
RR62_RS13250 (2695425)	2695425..2695754	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
RR62_RS13255 (2695754)	2695754..2696368	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
RR62_RS13260 (2696358)	2696358..2698349	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
RR62_RS13265 (2698371)	2698371..2699318	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T98295 WP_000227784.1 NZ_CP027361:c2694043-2693501 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T98295 NZ_CP027361:c2694043-2693501 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT98295 WP_001297137.1 NZ_CP027361:c2694338-2694027 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT98295 NZ_CP027361:c2694338-2694027 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3