Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1836946..1837443 Replicon chromosome
Accession NZ_CP027361
Organism Escherichia coli strain 2014C-4639

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag RR62_RS09085 Protein ID WP_001173343.1
Coordinates 1837270..1837443 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag RR62_RS09080 Protein ID WP_000398619.1
Coordinates 1836946..1837242 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR62_RS09060 (1833677) 1833677..1834396 - 720 WP_000611288.1 two-component system response regulator DcuR -
RR62_RS09065 (1834393) 1834393..1836024 - 1632 WP_001216487.1 sensor histidine kinase -
RR62_RS09070 (1836205) 1836205..1836435 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
RR62_RS09075 (1836447) 1836447..1836719 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
RR62_RS09080 (1836946) 1836946..1837242 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
RR62_RS09085 (1837270) 1837270..1837443 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
RR62_RS09090 (1837562) 1837562..1839079 - 1518 WP_001295074.1 lysine--tRNA ligase -
RR62_RS09095 (1839316) 1839316..1840773 - 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T98289 WP_001173343.1 NZ_CP027361:1837270-1837443 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T98289 NZ_CP027361:1837270-1837443 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT98289 WP_000398619.1 NZ_CP027361:1836946-1837242 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT98289 NZ_CP027361:1836946-1837242 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References