Detailed information of TA system TA098283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 121366..121991
• Replicon: plasmid unnamed2
• Accession: NZ_CP027357
• Organism: Escherichia coli strain 2013C-4991

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6P55_RS30020
• Protein ID: WP_000911324.1
• Coordinates: 121366..121764 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: C6P55_RS30025
• Protein ID: WP_000450532.1
• Coordinates: 121764..121991 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P55_RS29995	116664..118430	+	1767	Protein_129	type IV conjugative transfer system coupling protein TraD	-
C6P55_RS30000	118517..118942	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
C6P55_RS30005	118939..119289	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
C6P55_RS30010	119320..120933	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
C6P55_RS30015	120965..121357	+	393	Protein_133	conjugal transfer protein TraD	-
C6P55_RS30020	121366..121764	-	399	WP_000911324.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6P55_RS30025	121764..121991	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sitABCD	iroB / iroC / iroD / iroE / iroN / iroN	1..131463	131463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14800.09 Da
• Isoelectric Point: 7.8604

>T98283 WP_000911324.1 NZ_CP027357:c121764-121366 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T98283 NZ_CP027357:c121764-121366 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGGTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCTTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT98283 WP_000450532.1 NZ_CP027357:c121991-121764 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT98283 NZ_CP027357:c121991-121764 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5