Detailed information of TA system TA098277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 9178..9725
• Replicon: plasmid unnamed2
• Accession: NZ_CP027357
• Organism: Escherichia coli strain 2013C-4991

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: C6P55_RS29270
• Protein ID: WP_024170920.1
• Coordinates: 9444..9725 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A075M9N8
• Locus tag: C6P55_RS29265
• Protein ID: WP_032072813.1
• Coordinates: 9178..9447 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P55_RS29235	5206..6090	-	885	WP_000058717.1	EamA family transporter	-
C6P55_RS29240	6228..6635	-	408	WP_001214976.1	cysteine hydrolase	-
C6P55_RS29245	6624..8375	+	1752	Protein_10	Tn3-like element TnAs1 family transposase	-
C6P55_RS29265	9178..9447	+	270	WP_032072813.1	prevent host death protein Phd antitoxin	Antitoxin
C6P55_RS29270	9444..9725	+	282	WP_024170920.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6P55_RS29275	9771..10619	+	849	WP_032072812.1	3'-5' exonuclease	-
C6P55_RS29280	10736..11218	+	483	WP_001311056.1	hypothetical protein	-
C6P55_RS29290	11520..11867	+	348	WP_000142450.1	hypothetical protein	-
C6P55_RS31305	11885..12046	-	162	WP_000251882.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sitABCD	iroB / iroC / iroD / iroE / iroN / iroN	1..131463	131463
	-	flank	IS/Tn	tet(A)	-	3975..8375	4400

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10920.59 Da
• Isoelectric Point: 8.4615

>T98277 WP_024170920.1 NZ_CP027357:9444-9725 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T98277 NZ_CP027357:9444-9725 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGATAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10277.64 Da
• Isoelectric Point: 10.2879

>AT98277 WP_032072813.1 NZ_CP027357:9178-9447 [Escherichia coli]
MAQVNMNLRINAEIKNAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT98277 NZ_CP027357:9178-9447 [Escherichia coli]
ATGGCACAGGTTAATATGAACTTAAGAATCAACGCTGAAATAAAGAATGCTTTTATGGCTGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAACGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A075M9N8