Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35688..36331 Replicon plasmid unnamed1
Accession NZ_CP027356
Organism Escherichia coli strain 2013C-4991

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C6P55_RS28900 Protein ID WP_001044768.1
Coordinates 35915..36331 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C6P55_RS28895 Protein ID WP_001261287.1
Coordinates 35688..35918 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P55_RS28855 31554..32993 + 1440 WP_033803310.1 enterohemolysin T1SS ABC transporter subunit EhxD -
C6P55_RS28860 33060..33203 + 144 Protein_27 transcriptional regulator -
C6P55_RS28865 33207..33398 + 192 Protein_28 lipid II-degrading bacteriocin -
C6P55_RS28870 33406..33759 - 354 WP_000864810.1 colicin M immunity protein -
C6P55_RS28875 33932..34714 - 783 WP_001164205.1 site-specific integrase -
C6P55_RS28880 34716..35129 - 414 WP_000465041.1 hypothetical protein -
C6P55_RS28895 35688..35918 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6P55_RS28900 35915..36331 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C6P55_RS28905 36444..38015 - 1572 WP_000381383.1 IS66 family transposase -
C6P55_RS28910 38035..38382 - 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
C6P55_RS28915 38382..39059 - 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
C6P55_RS28925 39200..39745 - 546 WP_001165114.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / hlyC / hlyA / hlyB / hlyB / hlyD 1..71714 71714


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T98275 WP_001044768.1 NZ_CP027356:35915-36331 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T98275 NZ_CP027356:35915-36331 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT98275 WP_001261287.1 NZ_CP027356:35688-35918 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT98275 NZ_CP027356:35688-35918 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References