Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3003282..3003858 Replicon chromosome
Accession NZ_CP027355
Organism Escherichia coli strain 2013C-4991

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag C6P55_RS16440 Protein ID WP_001297643.1
Coordinates 3003571..3003858 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag C6P55_RS16435 Protein ID WP_000063156.1
Coordinates 3003282..3003584 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P55_RS16420 2999878..3002028 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
C6P55_RS16425 3002078..3002281 + 204 WP_000467859.1 YbdD/YjiX family protein -
C6P55_RS16430 3002292..3003248 + 957 WP_001297640.1 GTPase -
C6P55_RS16435 3003282..3003584 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
C6P55_RS16440 3003571..3003858 - 288 WP_001297643.1 BrnT family toxin Toxin
C6P55_RS16445 3004057..3004754 - 698 Protein_3001 IS1 family transposase -
C6P55_RS16450 3004811..3004906 + 96 Protein_3002 dienelactone hydrolase family protein -
C6P55_RS16455 3005052..3006284 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
C6P55_RS16460 3006325..3007604 + 1280 Protein_3004 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T98267 WP_001297643.1 NZ_CP027355:c3003858-3003571 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T98267 NZ_CP027355:c3003858-3003571 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT98267 WP_000063156.1 NZ_CP027355:c3003584-3003282 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT98267 NZ_CP027355:c3003584-3003282 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References