Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 8426..9069 Replicon plasmid unnamed2
Accession NZ_CP027354
Organism Escherichia coli strain 2012C-4606

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag AYN42_RS30810 Protein ID WP_001044768.1
Coordinates 8653..9069 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag AYN42_RS30805 Protein ID WP_001261287.1
Coordinates 8426..8656 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYN42_RS30760 4291..5730 + 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -
AYN42_RS30765 5797..5940 + 144 Protein_3 transcriptional regulator -
AYN42_RS30770 5944..6135 + 192 Protein_4 lipid II-degrading bacteriocin -
AYN42_RS30775 6143..6496 - 354 WP_000864810.1 colicin M immunity protein -
AYN42_RS30780 6669..7451 - 783 WP_001164205.1 site-specific integrase -
AYN42_RS30785 7453..7866 - 414 WP_000465041.1 hypothetical protein -
AYN42_RS30805 8426..8656 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYN42_RS30810 8653..9069 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
AYN42_RS30815 9231..9776 - 546 WP_001165114.1 hypothetical protein -
AYN42_RS30820 9844..11000 + 1157 WP_085948186.1 IS3-like element IS600 family transposase -
AYN42_RS31725 11041..11190 - 150 WP_000955366.1 hypothetical protein -
AYN42_RS30825 11805..12665 - 861 WP_000704534.1 alpha/beta hydrolase -
AYN42_RS30830 12793..13179 + 387 WP_001066949.1 tyrosine-type recombinase/integrase -
AYN42_RS30835 13233..13907 + 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyA / hlyB / hlyB / hlyD / toxB / toxB / toxB / toxB 1..57720 57720


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T98249 WP_001044768.1 NZ_CP027354:8653-9069 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T98249 NZ_CP027354:8653-9069 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT98249 WP_001261287.1 NZ_CP027354:8426-8656 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT98249 NZ_CP027354:8426-8656 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References