Detailed information of TA system TA098248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 5632857..5633335
• Replicon: chromosome
• Accession: NZ_CP027352
• Organism: Escherichia coli strain 2012C-4606

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: AYN42_RS30505
• Protein ID: WP_001303876.1
• Coordinates: 5632857..5633144 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: AYN42_RS30510
• Protein ID: WP_000536233.1
• Coordinates: 5633144..5633335 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN42_RS30470	5628080..5630530	-	2451	WP_106875240.1	exonuclease	-
AYN42_RS30475	5630624..5630815	-	192	WP_001098307.1	DUF1482 family protein	-
AYN42_RS30480	5630812..5631000	-	189	WP_000413705.1	cell division inhibition protein DicB	-
AYN42_RS30490	5631568..5631777	+	210	WP_001133037.1	hypothetical protein	-
AYN42_RS30495	5631778..5632416	-	639	WP_000394543.1	hypothetical protein	-
AYN42_RS30500	5632428..5632580	-	153	WP_000380316.1	DUF1391 family protein	-
AYN42_RS30505	5632857..5633144	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AYN42_RS30510	5633144..5633335	-	192	WP_000536233.1	hypothetical protein	Antitoxin
AYN42_RS30515	5633363..5633764	-	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
AYN42_RS30520	5633873..5634145	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
AYN42_RS30525	5634129..5634554	+	426	WP_000693855.1	Rha family transcriptional regulator	-
AYN42_RS30530	5634761..5635216	-	456	WP_000273724.1	hypothetical protein	-
AYN42_RS30535	5635295..5636410	+	1116	WP_001205821.1	hypothetical protein	-
AYN42_RS30540	5636417..5637163	+	747	WP_000788742.1	ATP-binding protein	-
AYN42_RS30545	5637185..5637955	+	771	WP_000451007.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5619417..5639862	20445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T98248 WP_001303876.1 NZ_CP027352:c5633144-5632857 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T98248 NZ_CP027352:c5633144-5632857 [Escherichia coli]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT98248 WP_000536233.1 NZ_CP027352:c5633335-5633144 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT98248 NZ_CP027352:c5633335-5633144 [Escherichia coli]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA