Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4983218..4983836 Replicon chromosome
Accession NZ_CP027352
Organism Escherichia coli strain 2012C-4606

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AYN42_RS27055 Protein ID WP_001291435.1
Coordinates 4983218..4983436 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AYN42_RS27060 Protein ID WP_000344800.1
Coordinates 4983462..4983836 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYN42_RS27020 4978508..4979080 + 573 WP_000779826.1 lipoprotein -
AYN42_RS27025 4979111..4979422 - 312 WP_000409911.1 MGMT family protein -
AYN42_RS27035 4979800..4980153 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AYN42_RS27040 4980195..4981745 - 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AYN42_RS27045 4981909..4982379 - 471 WP_000136192.1 YlaC family protein -
AYN42_RS27050 4982495..4983046 - 552 WP_000102564.1 maltose O-acetyltransferase -
AYN42_RS27055 4983218..4983436 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AYN42_RS27060 4983462..4983836 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AYN42_RS27065 4984382..4987531 - 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
AYN42_RS27070 4987554..4988747 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98247 WP_001291435.1 NZ_CP027352:c4983436-4983218 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98247 NZ_CP027352:c4983436-4983218 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98247 WP_000344800.1 NZ_CP027352:c4983836-4983462 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98247 NZ_CP027352:c4983836-4983462 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References