Detailed information of TA system TA098234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2220375..2221000
• Replicon: chromosome
• Accession: NZ_CP027352
• Organism: Escherichia coli strain 2012C-4606

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AYN42_RS12775
• Protein ID: WP_000911330.1
• Coordinates: 2220375..2220773 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AYN42_RS12780
• Protein ID: WP_000450524.1
• Coordinates: 2220773..2221000 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN42_RS12755	2216253..2216453	+	201	WP_000383836.1	YpfN family protein	-
AYN42_RS12760	2216563..2217261	-	699	WP_000679812.1	esterase	-
AYN42_RS12765	2217335..2219350	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
AYN42_RS12770	2219365..2220228	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
AYN42_RS12775	2220375..2220773	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AYN42_RS12780	2220773..2221000	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AYN42_RS12785	2221154..2221867	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AYN42_RS12790	2222080..2223114	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
AYN42_RS12795	2223131..2224009	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AYN42_RS12800	2224155..2224727	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
AYN42_RS12805	2224727..2225197	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T98234 WP_000911330.1 NZ_CP027352:c2220773-2220375 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98234 NZ_CP027352:c2220773-2220375 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98234 WP_000450524.1 NZ_CP027352:c2221000-2220773 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98234 NZ_CP027352:c2221000-2220773 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33