Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1386847..1387318 Replicon chromosome
Accession NZ_CP027352
Organism Escherichia coli strain 2012C-4606

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A1Y2Y8G6
Locus tag AYN42_RS08365 Protein ID WP_001448501.1
Coordinates 1387040..1387318 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag AYN42_RS08360 Protein ID WP_001302048.1
Coordinates 1386847..1387038 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYN42_RS08310 1381925..1382143 - 219 WP_000683609.1 DUF4014 family protein -
AYN42_RS08315 1382271..1382582 - 312 WP_001289673.1 hypothetical protein -
AYN42_RS08320 1382575..1382802 - 228 WP_000699809.1 hypothetical protein -
AYN42_RS08325 1382799..1383080 - 282 WP_000603384.1 DNA-binding protein -
AYN42_RS08330 1383113..1383874 - 762 WP_000450872.1 DUF1627 domain-containing protein -
AYN42_RS08335 1383900..1384641 - 742 Protein_1525 ATP-binding protein -
AYN42_RS08340 1384648..1385610 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
AYN42_RS08345 1385633..1386058 - 426 WP_000693943.1 Rha family transcriptional regulator -
AYN42_RS08350 1386055..1386357 - 303 WP_000172738.1 transcriptional regulator -
AYN42_RS08355 1386455..1386826 + 372 WP_001169687.1 hypothetical protein -
AYN42_RS08360 1386847..1387038 + 192 WP_001302048.1 hypothetical protein Antitoxin
AYN42_RS08365 1387040..1387318 + 279 WP_001448501.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AYN42_RS08370 1387605..1387757 + 153 WP_001427316.1 DUF1391 family protein -
AYN42_RS08375 1388178..1388399 + 222 WP_000560218.1 killing protein KilR -
AYN42_RS08380 1388393..1388569 + 177 WP_000245534.1 hypothetical protein -
AYN42_RS08385 1388643..1388918 + 276 WP_001307773.1 hypothetical protein -
AYN42_RS08390 1389017..1391668 + 2652 WP_000105101.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 1340496..1400195 59699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10691.27 Da        Isoelectric Point: 5.5647

>T98223 WP_001448501.1 NZ_CP027352:1387040-1387318 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPSLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98223 NZ_CP027352:1387040-1387318 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTAGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98223 WP_001302048.1 NZ_CP027352:1386847-1387038 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98223 NZ_CP027352:1386847-1387038 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y2Y8G6

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References