Detailed information of TA system TA098216

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5109175..5110012
• Replicon: chromosome
• Accession: NZ_CP027351
• Organism: Escherichia coli strain 2014C-3655

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7U9LL67
• Locus tag: ND05_RS27585
• Protein ID: WP_000854687.1
• Coordinates: 5109175..5109555 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ND05_RS27590
• Protein ID: WP_072189089.1
• Coordinates: 5109644..5110012 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ND05_RS27555	5104817..5106658	+	1842	WP_000437371.1	RNA polymerase sigma factor RpoD	-
ND05_RS27560	5106737..5107243	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
ND05_RS27570	5107543..5108388	-	846	WP_001274557.1	DUF4942 domain-containing protein	-
ND05_RS27575	5108473..5108670	-	198	WP_000772026.1	DUF957 domain-containing protein	-
ND05_RS27580	5108690..5109178	-	489	WP_001054228.1	hypothetical protein	-
ND05_RS27585	5109175..5109555	-	381	WP_000854687.1	TA system toxin CbtA family protein	Toxin
ND05_RS27590	5109644..5110012	-	369	WP_072189089.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ND05_RS27595	5110088..5110309	-	222	WP_000692312.1	DUF987 domain-containing protein	-
ND05_RS27600	5110372..5110848	-	477	WP_001186728.1	RadC family protein	-
ND05_RS27605	5110864..5111337	-	474	WP_000855068.1	antirestriction protein	-
ND05_RS27610	5111431..5111676	-	246	WP_001164974.1	hypothetical protein	-
ND05_RS27615	5111676..5112116	-	441	Protein_4958	DUF932 domain-containing protein	-
ND05_RS27620	5112235..5114013	-	1779	WP_001243192.1	reverse transcriptase/maturase family protein	-
ND05_RS27625	5114523..5114906	-	384	Protein_4960	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14199.13 Da
• Isoelectric Point: 6.8615

>T98216 WP_000854687.1 NZ_CP027351:c5109555-5109175 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNNITLGRHPEEAKQ

Nucleotide

• Length: 381 bp

>T98216 NZ_CP027351:c5109555-5109175 [Escherichia coli]
ATGAAAACATTACCCGACACTCACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCTCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATCCTCCGGGCCCGCAGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTAGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13723.54 Da
• Isoelectric Point: 5.9540

>AT98216 WP_072189089.1 NZ_CP027351:c5110012-5109644 [Escherichia coli]
VSDTLHETNYPNDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPDMKK

Nucleotide

• Length: 369 bp

>AT98216 NZ_CP027351:c5110012-5109644 [Escherichia coli]
GTGTCAGACACACTCCATGAGACAAATTATCCCAACGACAATCACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGATATGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LL67