Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4934667..4935465 Replicon chromosome
Accession NZ_CP027351
Organism Escherichia coli strain 2014C-3655

Toxin (Protein)


Gene name yeeV Uniprot ID A0A3W5Y7S6
Locus tag ND05_RS26595 Protein ID WP_000854916.1
Coordinates 4935088..4935465 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A3W5Y7S1
Locus tag ND05_RS26590 Protein ID WP_024174207.1
Coordinates 4934667..4935041 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND05_RS26550 4930245..4930487 - 243 WP_096855415.1 transposase -
ND05_RS29945 4930729..4930998 + 270 Protein_4760 transposase -
ND05_RS29950 4931003..4931469 - 467 Protein_4761 transposase -
ND05_RS26560 4931507..4931860 + 354 Protein_4762 transposase -
ND05_RS26565 4931992..4932417 + 426 WP_000422707.1 IS66 family insertion sequence hypothetical protein -
ND05_RS26570 4932414..4932764 + 351 WP_000624681.1 IS66 family insertion sequence element accessory protein TnpB -
ND05_RS26575 4932842..4934039 - 1198 Protein_4765 IS3 family transposase -
ND05_RS26580 4934080..4934214 + 135 Protein_4766 hypothetical protein -
ND05_RS26585 4934283..4934504 + 222 WP_000692312.1 DUF987 domain-containing protein -
ND05_RS26590 4934667..4935041 + 375 WP_024174207.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ND05_RS26595 4935088..4935465 + 378 WP_000854916.1 TA system toxin CbtA family protein Toxin
ND05_RS26600 4935462..4935950 + 489 WP_000777666.1 hypothetical protein -
ND05_RS26605 4935962..4936159 + 198 WP_000839246.1 DUF957 domain-containing protein -
ND05_RS30120 4936244..4936402 + 159 WP_000014512.1 hypothetical protein -
ND05_RS26610 4936420..4937976 - 1557 WP_001066422.1 IS66 family transposase -
ND05_RS26615 4937996..4938343 - 348 WP_000631725.1 IS66 family insertion sequence element accessory protein TnpB -
ND05_RS26620 4938340..4939014 - 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -
ND05_RS26625 4939090..4939803 + 714 Protein_4776 DUF4942 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espK / espF / escG / escF / cesD2 / espB / espD / espA / sepL / escD / eae 4923507..4956095 32588
- inside IScluster/Tn - - 4925975..4941305 15330


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14033.04 Da        Isoelectric Point: 9.1378

>T98214 WP_000854916.1 NZ_CP027351:4935088-4935465 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIKAGISLCDAVNFLVEKYALVRTDQPGF
SAGTSSQLINSIDILRARRATGLMTRSNYRTVNNITPGKHPEAKQ

Download         Length: 378 bp

>T98214 NZ_CP027351:4935088-4935465 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTA
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCCGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13789.59 Da        Isoelectric Point: 6.7387

>AT98214 WP_024174207.1 NZ_CP027351:4934667-4935041 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLTIYPTPATATTS

Download         Length: 375 bp

>AT98214 NZ_CP027351:4934667-4935041 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTATTTATCCGACACCGGCAACCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5Y7S6

Antitoxin

Source ID Structure
AlphaFold DB A0A3W5Y7S1

References