Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3030814..3031452 Replicon chromosome
Accession NZ_CP027351
Organism Escherichia coli strain 2014C-3655

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ND05_RS16555 Protein ID WP_000813794.1
Coordinates 3030814..3030990 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ND05_RS16560 Protein ID WP_001270286.1
Coordinates 3031036..3031452 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND05_RS16535 3026433..3027647 - 1215 WP_001326689.1 BenE family transporter YdcO -
ND05_RS16540 3027700..3028236 + 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
ND05_RS16545 3028309..3030270 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ND05_RS16550 3030362..3030592 - 231 WP_000494244.1 YncJ family protein -
ND05_RS16555 3030814..3030990 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ND05_RS16560 3031036..3031452 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ND05_RS16565 3031531..3032937 + 1407 WP_000760614.1 PLP-dependent aminotransferase family protein -
ND05_RS16570 3033182..3034327 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
ND05_RS16575 3034345..3035358 + 1014 WP_000220396.1 ABC transporter ATP-binding protein -
ND05_RS16580 3035359..3036300 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T98200 WP_000813794.1 NZ_CP027351:3030814-3030990 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T98200 NZ_CP027351:3030814-3030990 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT98200 WP_001270286.1 NZ_CP027351:3031036-3031452 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT98200 NZ_CP027351:3031036-3031452 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCACCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References