Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2557323..2557794 Replicon chromosome
Accession NZ_CP027351
Organism Escherichia coli strain 2014C-3655

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ND05_RS13720 Protein ID WP_001303511.1
Coordinates 2557323..2557601 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ND05_RS13725 Protein ID WP_001302048.1
Coordinates 2557603..2557794 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND05_RS13690 2552811..2553080 - 270 WP_000003742.1 excisionase -
ND05_RS13695 2553142..2555613 - 2472 WP_000048560.1 exonuclease -
ND05_RS13700 2555737..2555958 - 222 WP_000560212.1 cell division protein FtsZ -
ND05_RS13710 2556671..2556970 - 300 WP_001240334.1 hypothetical protein -
ND05_RS13720 2557323..2557601 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ND05_RS13725 2557603..2557794 - 192 WP_001302048.1 hypothetical protein Antitoxin
ND05_RS13730 2557815..2558186 - 372 WP_001169687.1 hypothetical protein -
ND05_RS13735 2558284..2558586 + 303 WP_000172738.1 transcriptional regulator -
ND05_RS13740 2558583..2559008 + 426 WP_000693944.1 Rha family transcriptional regulator -
ND05_RS13745 2559031..2559993 + 963 WP_000095671.1 helix-turn-helix domain-containing protein -
ND05_RS13750 2560034..2560456 + 423 WP_001151187.1 DUF977 family protein -
ND05_RS13755 2560562..2560774 + 213 WP_000935423.1 hypothetical protein -
ND05_RS13760 2560807..2561025 + 219 WP_000209148.1 DUF4014 family protein -
ND05_RS29655 2561027..2561185 + 159 WP_001370098.1 hypothetical protein -
ND05_RS13765 2561189..2562745 - 1557 WP_001066422.1 IS66 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2547876..2594003 46127


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T98195 WP_001303511.1 NZ_CP027351:c2557601-2557323 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98195 NZ_CP027351:c2557601-2557323 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98195 WP_001302048.1 NZ_CP027351:c2557794-2557603 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98195 NZ_CP027351:c2557794-2557603 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References