Detailed information of TA system TA098172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2622179..2622804
• Replicon: chromosome
• Accession: NZ_CP027347
• Organism: Escherichia coli strain 2013C-4361

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AZF34_RS14405
• Protein ID: WP_000911330.1
• Coordinates: 2622179..2622577 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: AZF34_RS14410
• Protein ID: WP_000450524.1
• Coordinates: 2622577..2622804 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZF34_RS14385	2618057..2618257	+	201	WP_000383836.1	YpfN family protein	-
AZF34_RS14390	2618367..2619065	-	699	WP_000679812.1	esterase	-
AZF34_RS14395	2619139..2621154	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
AZF34_RS14400	2621169..2622032	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
AZF34_RS14405	2622179..2622577	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
AZF34_RS14410	2622577..2622804	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
AZF34_RS14415	2622958..2623671	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
AZF34_RS14420	2623884..2624918	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
AZF34_RS14425	2624935..2625813	-	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
AZF34_RS14430	2625959..2626543	+	585	WP_000176194.1	glycine cleavage system transcriptional repressor	-
AZF34_RS14435	2626543..2627013	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T98172 WP_000911330.1 NZ_CP027347:c2622577-2622179 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98172 NZ_CP027347:c2622577-2622179 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98172 WP_000450524.1 NZ_CP027347:c2622804-2622577 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98172 NZ_CP027347:c2622804-2622577 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33