Detailed information of TA system TA098161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1328227..1328865
• Replicon: chromosome
• Accession: NZ_CP027347
• Organism: Escherichia coli strain 2013C-4361

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: AZF34_RS07315
• Protein ID: WP_000813794.1
• Coordinates: 1328227..1328403 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: AZF34_RS07320
• Protein ID: WP_001270286.1
• Coordinates: 1328449..1328865 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZF34_RS07295	1323852..1325060	-	1209	WP_071532666.1	BenE family transporter YdcO	-
AZF34_RS07300	1325113..1325649	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
AZF34_RS07305	1325722..1327683	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
AZF34_RS07310	1327775..1328005	-	231	WP_000494244.1	YncJ family protein	-
AZF34_RS07315	1328227..1328403	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
AZF34_RS07320	1328449..1328865	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
AZF34_RS07325	1328944..1330350	+	1407	WP_000760637.1	PLP-dependent aminotransferase family protein	-
AZF34_RS07330	1330595..1331740	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
AZF34_RS07335	1331758..1332771	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
AZF34_RS07340	1332772..1333713	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1322664..1323812	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T98161 WP_000813794.1 NZ_CP027347:1328227-1328403 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T98161 NZ_CP027347:1328227-1328403 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT98161 WP_001270286.1 NZ_CP027347:1328449-1328865 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT98161 NZ_CP027347:1328449-1328865 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB