Detailed information of TA system TA098155

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 809517..810312
• Replicon: chromosome
• Accession: NZ_CP027347
• Organism: Escherichia coli strain 2013C-4361

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7UP43
• Locus tag: AZF34_RS04340
• Protein ID: WP_000854914.1
• Coordinates: 809938..810312 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0B0VS41
• Locus tag: AZF34_RS04335
• Protein ID: WP_001280954.1
• Coordinates: 809517..809891 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZF34_RS04300	806524..806844	+	321	WP_000149392.1	phospholipase	-
AZF34_RS04315	807184..808002	+	819	WP_001234682.1	DUF945 domain-containing protein	-
AZF34_RS04320	808093..808578	+	486	WP_000214398.1	antirestriction protein	-
AZF34_RS04325	808594..809070	+	477	WP_001345397.1	RadC family protein	-
AZF34_RS04330	809133..809354	+	222	WP_001220314.1	DUF987 domain-containing protein	-
AZF34_RS04335	809517..809891	+	375	WP_001280954.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
AZF34_RS04340	809938..810312	+	375	WP_000854914.1	TA system toxin CbtA family protein	Toxin
AZF34_RS04345	810309..810800	+	492	WP_000976857.1	hypothetical protein	-
AZF34_RS04350	810812..811009	+	198	WP_000839282.1	DUF957 domain-containing protein	-
AZF34_RS04355	811094..811936	+	843	WP_001280481.1	DUF4942 domain-containing protein	-
AZF34_RS04365	812407..813345	+	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
AZF34_RS04370	813400..814137	+	738	WP_000283664.1	zinc-binding phosphatase	-
AZF34_RS04375	814161..814715	+	555	WP_001001902.1	molecular chaperone YcdY	-
AZF34_RS04380	814817..815308	+	492	WP_001297187.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgG / csgF / csgE / csgD / csgB / csgA / csgC	808594..820873	12279

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14117.17 Da
• Isoelectric Point: 7.7761

>T98155 WP_000854914.1 NZ_CP027347:809938-810312 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T98155 NZ_CP027347:809938-810312 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13718.51 Da
• Isoelectric Point: 6.6249

>AT98155 WP_001280954.1 NZ_CP027347:809517-809891 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLNAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Nucleotide

• Length: 375 bp

>AT98155 NZ_CP027347:809517-809891 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGAATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LXR5

Antitoxin

• AlphaFold DB: A0A0B0VS41