Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 51341..51959 Replicon chromosome
Accession NZ_CP027347
Organism Escherichia coli strain 2013C-4361

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AZF34_RS00270 Protein ID WP_001291435.1
Coordinates 51341..51559 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AZF34_RS00275 Protein ID WP_000344800.1
Coordinates 51585..51959 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AZF34_RS00235 46630..47202 + 573 WP_000779826.1 lipoprotein -
AZF34_RS00240 47233..47544 - 312 WP_000409911.1 MGMT family protein -
AZF34_RS00250 47923..48276 + 354 WP_000878140.1 DUF1428 domain-containing protein -
AZF34_RS00255 48318..49868 - 1551 WP_001369170.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AZF34_RS00260 50032..50502 - 471 WP_001345013.1 YlaC family protein -
AZF34_RS00265 50618..51169 - 552 WP_000102564.1 maltose O-acetyltransferase -
AZF34_RS00270 51341..51559 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AZF34_RS00275 51585..51959 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AZF34_RS00280 52505..55654 - 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
AZF34_RS00285 55677..56870 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98154 WP_001291435.1 NZ_CP027347:c51559-51341 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98154 NZ_CP027347:c51559-51341 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98154 WP_000344800.1 NZ_CP027347:c51959-51585 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98154 NZ_CP027347:c51959-51585 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References