Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 46207..46732 Replicon plasmid unnamed
Accession NZ_CP027343
Organism Escherichia coli strain 2014C-4587

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6N23_RS26485 Protein ID WP_001159871.1
Coordinates 46207..46512 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6N23_RS26490 Protein ID WP_000813639.1
Coordinates 46514..46732 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N23_RS26450 41474..42744 + 1271 Protein_61 translesion error-prone DNA polymerase V subunit UmuC -
C6N23_RS26460 42749..43141 - 393 WP_000340836.1 plasmid partitioning/stability family protein -
C6N23_RS26465 43146..44117 - 972 WP_001103695.1 hypothetical protein -
C6N23_RS26470 44346..44990 + 645 WP_000633912.1 AAA family ATPase -
C6N23_RS26475 44984..45259 + 276 WP_000239527.1 hypothetical protein -
C6N23_RS26480 45397..46206 - 810 WP_000016958.1 site-specific integrase -
C6N23_RS26485 46207..46512 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6N23_RS26490 46514..46732 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6N23_RS26495 47328..47588 + 261 WP_000343084.1 hypothetical protein -
C6N23_RS26500 47585..48175 + 591 WP_000194564.1 hypothetical protein -
C6N23_RS27340 48193..48540 - 348 WP_000142428.1 hypothetical protein -
C6N23_RS26515 48725..49003 + 279 WP_001365566.1 hypothetical protein -
C6N23_RS26520 49010..49345 - 336 Protein_73 colicin -
C6N23_RS26525 49440..50171 - 732 WP_000850413.1 complement resistance protein TraT -
C6N23_RS27345 50405..50621 - 217 Protein_75 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / espP 1..131410 131410


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T98149 WP_001159871.1 NZ_CP027343:c46512-46207 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T98149 NZ_CP027343:c46512-46207 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCTGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT98149 WP_000813639.1 NZ_CP027343:c46732-46514 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98149 NZ_CP027343:c46732-46514 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References