Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1718577..1719231 Replicon chromosome
Accession NZ_CP027342
Organism Escherichia coli strain 2014C-4587

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag C6N23_RS08815 Protein ID WP_000244781.1
Coordinates 1718824..1719231 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag C6N23_RS08810 Protein ID WP_000354046.1
Coordinates 1718577..1718843 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N23_RS08785 1713746..1714489 + 744 WP_000951961.1 SDR family oxidoreductase -
C6N23_RS08790 1714546..1715979 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
C6N23_RS08795 1716024..1716335 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
C6N23_RS08800 1716499..1717158 + 660 WP_000250274.1 hemolysin III family protein -
C6N23_RS08805 1717354..1718334 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
C6N23_RS08810 1718577..1718843 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
C6N23_RS08815 1718824..1719231 + 408 WP_000244781.1 protein YgfX Toxin
C6N23_RS08820 1719271..1719792 - 522 WP_001055874.1 flavodoxin FldB -
C6N23_RS08825 1719904..1720800 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
C6N23_RS08830 1720825..1721535 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
C6N23_RS08835 1721541..1723274 + 1734 WP_000813218.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T98130 WP_000244781.1 NZ_CP027342:1718824-1719231 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T98130 NZ_CP027342:1718824-1719231 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT98130 WP_000354046.1 NZ_CP027342:1718577-1718843 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT98130 NZ_CP027342:1718577-1718843 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References