Detailed information of TA system TA098123

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 118633..119411
• Replicon: chromosome
• Accession: NZ_CP027342
• Organism: Escherichia coli strain 2014C-4587

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A3U1V6G9
• Locus tag: C6N23_RS00600
• Protein ID: WP_033805147.1
• Coordinates: 118633..119007 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3U1V6I4
• Locus tag: C6N23_RS00605
• Protein ID: WP_033805146.1
• Coordinates: 119046..119411 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N23_RS00585	114791..115204	+	414	WP_001368082.1	hypothetical protein	-
C6N23_RS00590	115201..116670	+	1470	WP_001022619.1	serine/threonine protein kinase	-
C6N23_RS00595	116872..118136	-	1265	Protein_105	tyrosine-type recombinase/integrase	-
C6N23_RS00600	118633..119007	-	375	WP_033805147.1	TA system toxin CbtA family protein	Toxin
C6N23_RS00605	119046..119411	-	366	WP_033805146.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6N23_RS00610	119437..119658	-	222	WP_033805145.1	DUF987 domain-containing protein	-
C6N23_RS00615	119655..120197	-	543	WP_033805144.1	DNA repair protein RadC	-
C6N23_RS00620	120210..120650	-	441	WP_033805143.1	antirestriction protein	-
C6N23_RS00625	120681..121502	-	822	WP_047082063.1	DUF945 domain-containing protein	-
C6N23_RS00630	121604..121834	-	231	WP_033805141.1	DUF905 domain-containing protein	-
C6N23_RS00635	121906..122355	-	450	WP_033805140.1	hypothetical protein	-
C6N23_RS00640	122352..122804	-	453	WP_033805139.1	hypothetical protein	-
C6N23_RS00645	122841..123410	-	570	WP_033805138.1	hypothetical protein	-
C6N23_RS00650	123410..124114	-	705	WP_033805137.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	88244..129525	41281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13843.88 Da
• Isoelectric Point: 7.9537

>T98123 WP_033805147.1 NZ_CP027342:c119007-118633 [Escherichia coli]
MQTISSHLTRAAQPCLSPVEIWQLLLTHLLSQHYGLTLNDTPFSDETTIQKHIDAGISLSDAVNFLVEKYELIRIDRDGC
SVMAQKSPFITSIDILRARKASGLMKRSSHKTVTRATAGQHQEL

Nucleotide

• Length: 375 bp

>T98123 NZ_CP027342:c119007-118633 [Escherichia coli]
ATGCAAACCATATCATCACACCTGACACGGGCGGCTCAGCCCTGCCTGTCACCCGTTGAAATCTGGCAGTTGCTGCTGAC
TCATCTGTTATCGCAGCACTATGGCCTGACGCTGAATGACACACCGTTCAGTGATGAAACCACCATCCAGAAACATATTG
ATGCAGGAATATCGCTCAGCGATGCGGTGAACTTTCTTGTCGAAAAATATGAACTGATAAGAATTGACCGCGATGGTTGC
TCTGTCATGGCGCAGAAGTCACCGTTTATCACCAGTATCGATATTCTACGTGCCCGGAAAGCCTCCGGTCTGATGAAACG
CAGCAGTCACAAAACTGTTACCAGGGCAACGGCAGGCCAACATCAGGAGCTATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13393.06 Da
• Isoelectric Point: 5.0700

>AT98123 WP_033805146.1 NZ_CP027342:c119411-119046 [Escherichia coli]
MNNHSESGAIPENPPCQQWGLKSTITPCFGARLVQEGDRLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELMLTSGE
LNPRHPHSVTLYHNGLTCEADTLGSCGYVFIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT98123 NZ_CP027342:c119411-119046 [Escherichia coli]
ATGAACAATCACTCAGAATCCGGTGCCATACCGGAGAATCCCCCCTGCCAGCAGTGGGGGCTAAAGAGTACGATCACACC
CTGTTTTGGAGCTCGGCTGGTGCAAGAAGGCGACCGCCTGCATTTTCTCGCTGACCGCGCCGGGTTTAACGGTGCTTTCA
GCGACGAGCATGCGTTGCGCCTAGACCAGGCCTTTCCGTTGATACTCAAGCAGCTGGAGTTGATGCTCACCAGCGGAGAG
CTCAACCCTAGGCACCCGCACAGCGTCACGCTTTACCACAACGGACTCACCTGCGAAGCCGACACACTCGGCTCCTGTGG
CTATGTCTTCATTGCTATCTATCCTGAGCAGACTGAGCCGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A3U1V6G9

Antitoxin

• AlphaFold DB: A0A3U1V6I4