Detailed information of TA system TA098122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 63584..64119
• Replicon: plasmid unnamed
• Accession: NZ_CP027341
• Organism: Escherichia coli strain 2015C-3121

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: C6N25_RS29625
• Protein ID: WP_000222760.1
• Coordinates: 63584..63871 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: C6N25_RS29630
• Protein ID: WP_001132900.1
• Coordinates: 63868..64119 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N25_RS29585	59029..59454	+	426	WP_001249909.1	nucleoside triphosphatase NudI	-
C6N25_RS29590	59512..59678	+	167	Protein_79	IS4 family transposase	-
C6N25_RS29595	59721..59942	-	222	Protein_80	DDE-type integrase/transposase/recombinase	-
C6N25_RS29600	60033..60398	+	366	WP_000091308.1	hypothetical protein	-
C6N25_RS29605	60398..61585	+	1188	WP_106888924.1	IS91 family transposase	-
C6N25_RS29610	61772..62062	-	291	WP_062892292.1	hypothetical protein	-
C6N25_RS29615	62130..62787	-	658	Protein_84	hypothetical protein	-
C6N25_RS29620	62890..63480	-	591	WP_000977350.1	serine acetyltransferase	-
C6N25_RS29625	63584..63871	-	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N25_RS29630	63868..64119	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6N25_RS29645	65078..65950	-	873	WP_000729440.1	incFII family plasmid replication initiator RepA	-
C6N25_RS29660	66653..67294	+	642	WP_077746831.1	transcription termination factor NusG	-
C6N25_RS29665	67435..68100	+	666	WP_000154145.1	hypothetical protein	-
C6N25_RS29675	68366..68986	+	621	WP_000176497.1	DUF1449 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..104198	104198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T98122 WP_000222760.1 NZ_CP027341:c63871-63584 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T98122 NZ_CP027341:c63871-63584 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT98122 WP_001132900.1 NZ_CP027341:c64119-63868 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT98122 NZ_CP027341:c64119-63868 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2