Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 42401..42926 Replicon plasmid unnamed
Accession NZ_CP027341
Organism Escherichia coli strain 2015C-3121

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag C6N25_RS29470 Protein ID WP_001159871.1
Coordinates 42401..42706 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag C6N25_RS29475 Protein ID WP_000813639.1
Coordinates 42708..42926 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N25_RS29445 38573..38989 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
C6N25_RS29450 38982..39962 - 981 WP_000688509.1 plasmid segregation protein ParM -
C6N25_RS29455 40374..40682 - 309 WP_000030204.1 molecular chaperone GroEL -
C6N25_RS29460 40769..41413 - 645 WP_001144036.1 ParA family protein -
C6N25_RS29465 41594..42400 - 807 WP_000016973.1 site-specific integrase -
C6N25_RS29470 42401..42706 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
C6N25_RS29475 42708..42926 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6N25_RS29480 43773..45011 + 1239 WP_024178320.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
C6N25_RS29485 44995..45825 + 831 WP_016230897.1 HAD-IIB family hydrolase -
C6N25_RS29490 45825..46841 + 1017 WP_000583427.1 Gfo/Idh/MocA family oxidoreductase -
C6N25_RS29495 46843..47112 + 270 WP_000379710.1 SemiSWEET transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..104198 104198


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T98121 WP_001159871.1 NZ_CP027341:c42706-42401 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T98121 NZ_CP027341:c42706-42401 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT98121 WP_000813639.1 NZ_CP027341:c42926-42708 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98121 NZ_CP027341:c42926-42708 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAATGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References