Detailed information of TA system TA098120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5140485..5141110
• Replicon: chromosome
• Accession: NZ_CP027340
• Organism: Escherichia coli strain 2015C-3121

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: C6N25_RS27880
• Protein ID: WP_000911330.1
• Coordinates: 5140485..5140883 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: C6N25_RS27885
• Protein ID: WP_000450524.1
• Coordinates: 5140883..5141110 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N25_RS27860	5136363..5136563	+	201	WP_000383836.1	YpfN family protein	-
C6N25_RS27865	5136673..5137371	-	699	WP_000679812.1	esterase	-
C6N25_RS27870	5137445..5139460	-	2016	WP_000829394.1	tRNA cytosine(34) acetyltransferase TmcA	-
C6N25_RS27875	5139475..5140338	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
C6N25_RS27880	5140485..5140883	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
C6N25_RS27885	5140883..5141110	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
C6N25_RS27890	5141264..5141977	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
C6N25_RS27895	5142190..5143224	-	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
C6N25_RS27900	5143241..5144119	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
C6N25_RS27905	5144265..5144837	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
C6N25_RS27910	5144837..5145307	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T98120 WP_000911330.1 NZ_CP027340:c5140883-5140485 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T98120 NZ_CP027340:c5140883-5140485 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT98120 WP_000450524.1 NZ_CP027340:c5141110-5140883 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT98120 NZ_CP027340:c5141110-5140883 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33