Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3859090..3859728 Replicon chromosome
Accession NZ_CP027340
Organism Escherichia coli strain 2015C-3121

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag C6N25_RS20895 Protein ID WP_000813794.1
Coordinates 3859552..3859728 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag C6N25_RS20890 Protein ID WP_001270286.1
Coordinates 3859090..3859506 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N25_RS20865 3854407..3855420 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
C6N25_RS20870 3855438..3856583 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
C6N25_RS20875 3856853..3857550 - 698 Protein_3747 IS1-like element IS1A family transposase -
C6N25_RS20885 3857605..3859011 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
C6N25_RS20890 3859090..3859506 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
C6N25_RS20895 3859552..3859728 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
C6N25_RS20900 3859950..3860180 + 231 WP_000494244.1 YncJ family protein -
C6N25_RS20905 3860272..3862233 - 1962 WP_024178299.1 23S rRNA 5-hydroxycytidine C2501 synthase -
C6N25_RS20910 3862306..3862842 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
C6N25_RS20915 3862895..3864115 + 1221 WP_071997226.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3864155..3865420 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T98110 WP_000813794.1 NZ_CP027340:c3859728-3859552 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T98110 NZ_CP027340:c3859728-3859552 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT98110 WP_001270286.1 NZ_CP027340:c3859506-3859090 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT98110 NZ_CP027340:c3859506-3859090 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References