Detailed information of TA system TA098107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3291059..3291648
• Replicon: chromosome
• Accession: NZ_CP027340
• Organism: Escherichia coli strain 2015C-3121

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A398TMA1
• Locus tag: C6N25_RS17595
• Protein ID: WP_000513533.1
• Coordinates: 3291059..3291307 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A3J8R0E8
• Locus tag: C6N25_RS17600
• Protein ID: WP_000681611.1
• Coordinates: 3291304..3291648 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N25_RS17545	3286352..3286939	-	588	WP_000344637.1	hypothetical protein	-
C6N25_RS17550	3286939..3287628	-	690	WP_000187063.1	YqaJ viral recombinase family protein	-
C6N25_RS17560	3287792..3288489	-	698	Protein_3146	IS1-like element IS1A family transposase	-
C6N25_RS17570	3288546..3288629	-	84	Protein_3147	recombinase RecT	-
C6N25_RS17575	3288647..3288928	-	282	WP_000995348.1	host nuclease inhibitor GamL	-
C6N25_RS17580	3288949..3289230	-	282	WP_000934192.1	hypothetical protein	-
C6N25_RS17585	3289242..3289454	-	213	WP_000917251.1	hypothetical protein	-
C6N25_RS17590	3289525..3290307	-	783	WP_158707279.1	Rha family phage regulatory protein	-
C6N25_RS17595	3291059..3291307	+	249	WP_000513533.1	type II toxin-antitoxin system HicA family toxin	Toxin
C6N25_RS17600	3291304..3291648	+	345	WP_000681611.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
C6N25_RS30430	3291765..3291935	-	171	WP_167580584.1	hypothetical protein	-
C6N25_RS17605	3291936..3292838	-	903	WP_000081325.1	serine dehydrogenasease	-
C6N25_RS17610	3293018..3293179	-	162	WP_071525586.1	transmembrane anchored protein	-
C6N25_RS17615	3293276..3293989	-	714	WP_001056249.1	LexA family transcriptional regulator	-
C6N25_RS17620	3294085..3294288	+	204	WP_001240876.1	Cro/Cl family transcriptional regulator	-
C6N25_RS17625	3294459..3294653	+	195	WP_001302923.1	hypothetical protein	-
C6N25_RS17630	3294820..3295197	+	378	WP_001271433.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	stxA / stxB	3275461..3341759	66298

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9436.07 Da
• Isoelectric Point: 10.3195

>T98107 WP_000513533.1 NZ_CP027340:3291059-3291307 [Escherichia coli]
MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Nucleotide

• Length: 249 bp

>T98107 NZ_CP027340:3291059-3291307 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGAAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAGCTGGTCGTTTTGTT
TTCCTCTCTGGGATATGTCAAAAAGGAAATGCAGGGCTCAAGAGTGCGGTTTTTCAATGCTGAAATCAACCACACCATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCGATTAAACAGAATCTGAAAGAGGCTGGG
GTATTATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13065.80 Da
• Isoelectric Point: 4.6791

>AT98107 WP_000681611.1 NZ_CP027340:3291304-3291648 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELEQEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVNHHTGA

Nucleotide

• Length: 345 bp

>AT98107 NZ_CP027340:3291304-3291648 [Escherichia coli]
ATGAAGCATCTAAAATATAAAGGATATTTAGGTACAGTAGAACCAGATTTTGAAAATAATATCCTGTACGGAAAACTGGC
GTTTATTCGAGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAGACATCGGTTGAGCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAGCCTGATACCCCGTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCGCTGGAAAAAGA
AGTCAACCATCACACAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A398TMA1

Antitoxin

• AlphaFold DB: A0A3J8R0E8