Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2657625..2658243 Replicon chromosome
Accession NZ_CP027340
Organism Escherichia coli strain 2015C-3121

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag C6N25_RS14220 Protein ID WP_001291435.1
Coordinates 2657625..2657843 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag C6N25_RS14225 Protein ID WP_000344800.1
Coordinates 2657869..2658243 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N25_RS14185 2652914..2653486 + 573 WP_000779827.1 YbaY family lipoprotein -
C6N25_RS14190 2653517..2653828 - 312 WP_000409911.1 MGMT family protein -
C6N25_RS14200 2654207..2654560 + 354 WP_000878141.1 DUF1428 family protein -
C6N25_RS14205 2654602..2656152 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
C6N25_RS14210 2656316..2656786 - 471 WP_046122743.1 YlaC family protein -
C6N25_RS14215 2656902..2657453 - 552 WP_000102564.1 maltose O-acetyltransferase -
C6N25_RS14220 2657625..2657843 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
C6N25_RS14225 2657869..2658243 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
C6N25_RS14230 2658789..2661938 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
C6N25_RS14235 2661961..2663154 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98106 WP_001291435.1 NZ_CP027340:c2657843-2657625 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98106 NZ_CP027340:c2657843-2657625 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98106 WP_000344800.1 NZ_CP027340:c2658243-2657869 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98106 NZ_CP027340:c2658243-2657869 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References