Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 78803..79446 Replicon plasmid unnamed
Accession NZ_CP027339
Organism Escherichia coli strain 2014C-3051

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag C6N28_RS30685 Protein ID WP_001044768.1
Coordinates 79030..79446 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag C6N28_RS30680 Protein ID WP_001261287.1
Coordinates 78803..79033 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N28_RS30640 74906..75049 + 144 Protein_72 transcriptional regulator -
C6N28_RS30645 75053..75244 + 192 Protein_73 lipid II-degrading bacteriocin -
C6N28_RS30655 76525..76872 - 348 WP_106889347.1 colicin M immunity protein -
C6N28_RS30660 77045..77827 - 783 WP_001164205.1 site-specific integrase -
C6N28_RS30665 77829..78241 - 413 Protein_77 hypothetical protein -
C6N28_RS30680 78803..79033 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6N28_RS30685 79030..79446 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
C6N28_RS30690 79608..80153 - 546 WP_001165114.1 hypothetical protein -
C6N28_RS30695 80221..81377 + 1157 WP_085948186.1 IS3-like element IS600 family transposase -
C6N28_RS31180 81418..81693 - 276 WP_157776129.1 hypothetical protein -
C6N28_RS30700 82182..83042 - 861 WP_000704534.1 alpha/beta hydrolase -
C6N28_RS30705 83170..83556 + 387 WP_001066949.1 tyrosine-type recombinase/integrase -
C6N28_RS30710 83610..84284 + 675 WP_001341423.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - toxB / katA / espP / hlyC / hlyA / hlyA / hlyB / hlyD / toxB 1..92644 92644
- inside IScluster/Tn - - 75317..84628 9311


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T98096 WP_001044768.1 NZ_CP027339:79030-79446 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T98096 NZ_CP027339:79030-79446 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGCATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT98096 WP_001261287.1 NZ_CP027339:78803-79033 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT98096 NZ_CP027339:78803-79033 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References