Detailed information of TA system TA098090

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4682438..4683270
• Replicon: chromosome
• Accession: NZ_CP027338
• Organism: Escherichia coli strain 2014C-3051

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: C6N28_RS25480
• Protein ID: WP_000854753.1
• Coordinates: 4682896..4683270 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: V0ULY5
• Locus tag: C6N28_RS25475
• Protein ID: WP_001315620.1
• Coordinates: 4682438..4682806 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N28_RS25440	4679275..4679730	+	456	WP_000581504.1	hypothetical protein	-
C6N28_RS25445	4679809..4680042	+	234	WP_001119729.1	DUF905 family protein	-
C6N28_RS25455	4680142..4680959	+	818	Protein_4629	DUF932 domain-containing protein	-
C6N28_RS25460	4681014..4681499	+	486	WP_000849588.1	antirestriction protein	-
C6N28_RS25465	4681515..4681991	+	477	WP_001186774.1	RadC family protein	-
C6N28_RS25470	4682054..4682275	+	222	WP_000692345.1	DUF987 domain-containing protein	-
C6N28_RS25475	4682438..4682806	+	369	WP_001315620.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
C6N28_RS25480	4682896..4683270	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
C6N28_RS25485	4683267..4683755	+	489	WP_000777547.1	hypothetical protein	-
C6N28_RS25490	4683767..4683964	+	198	WP_000839281.1	DUF957 domain-containing protein	-
C6N28_RS31370	4684049..4684201	+	153	WP_001280445.1	hypothetical protein	-
C6N28_RS25515	4685246..4685896	+	651	WP_001037966.1	HNH endonuclease	-
C6N28_RS25520	4685904..4686884	-	981	WP_000991415.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
C6N28_RS25525	4686949..4688055	-	1107	WP_001341302.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T98090 WP_000854753.1 NZ_CP027338:4682896-4683270 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T98090 NZ_CP027338:4682896-4683270 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13474.20 Da
• Isoelectric Point: 6.2050

>AT98090 WP_001315620.1 NZ_CP027338:4682438-4682806 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT98090 NZ_CP027338:4682438-4682806 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: V0ULY5