Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1594479..1594950 Replicon chromosome
Accession NZ_CP027338
Organism Escherichia coli strain 2014C-3051

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag C6N28_RS09200 Protein ID WP_001303511.1
Coordinates 1594672..1594950 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag C6N28_RS09195 Protein ID WP_001302048.1
Coordinates 1594479..1594670 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N28_RS09145 1589557..1589775 - 219 WP_000683609.1 DUF4014 family protein -
C6N28_RS09150 1589903..1590214 - 312 WP_001289673.1 hypothetical protein -
C6N28_RS09155 1590207..1590434 - 228 WP_000699809.1 hypothetical protein -
C6N28_RS09160 1590431..1590712 - 282 WP_000603384.1 DNA-binding protein -
C6N28_RS09165 1590745..1591506 - 762 WP_000450872.1 DUF1627 domain-containing protein -
C6N28_RS09170 1591532..1592273 - 742 Protein_1677 ATP-binding protein -
C6N28_RS09175 1592280..1593242 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
C6N28_RS09180 1593265..1593690 - 426 WP_000693943.1 Rha family transcriptional regulator -
C6N28_RS09185 1593687..1593989 - 303 WP_000172738.1 transcriptional regulator -
C6N28_RS09190 1594087..1594458 + 372 WP_001169687.1 hypothetical protein -
C6N28_RS09195 1594479..1594670 + 192 WP_001302048.1 hypothetical protein Antitoxin
C6N28_RS09200 1594672..1594950 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6N28_RS09205 1595237..1595389 + 153 WP_001427316.1 DUF1391 family protein -
C6N28_RS09210 1595810..1596031 + 222 WP_000560218.1 killing protein KilR -
C6N28_RS09215 1596025..1596201 + 177 WP_000245534.1 hypothetical protein -
C6N28_RS09220 1596275..1596550 + 276 WP_001307773.1 hypothetical protein -
C6N28_RS09225 1596649..1598478 + 1830 Protein_1688 exonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 1549721..1609098 59377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T98073 WP_001303511.1 NZ_CP027338:1594672-1594950 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T98073 NZ_CP027338:1594672-1594950 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98073 WP_001302048.1 NZ_CP027338:1594479-1594670 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98073 NZ_CP027338:1594479-1594670 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References