Detailed information of TA system TA098071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1088843..1089481
• Replicon: chromosome
• Accession: NZ_CP027338
• Organism: Escherichia coli strain 2014C-3051

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: C6N28_RS06350
• Protein ID: WP_000813794.1
• Coordinates: 1088843..1089019 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: C6N28_RS06355
• Protein ID: WP_001270286.1
• Coordinates: 1089065..1089481 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N28_RS06330	1084456..1085676	-	1221	WP_071997226.1	BenE family transporter YdcO	-
C6N28_RS06335	1085729..1086265	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
C6N28_RS06340	1086338..1088299	+	1962	WP_001341357.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
C6N28_RS06345	1088391..1088621	-	231	WP_000494244.1	YncJ family protein	-
C6N28_RS06350	1088843..1089019	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
C6N28_RS06355	1089065..1089481	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
C6N28_RS06360	1089560..1090965	+	1406	Protein_1166	PLP-dependent aminotransferase family protein	-
C6N28_RS06365	1091210..1092355	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
C6N28_RS06370	1092373..1093386	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
C6N28_RS06375	1093387..1094306	+	920	Protein_1169	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1083268..1084416	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T98071 WP_000813794.1 NZ_CP027338:1088843-1089019 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T98071 NZ_CP027338:1088843-1089019 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT98071 WP_001270286.1 NZ_CP027338:1089065-1089481 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT98071 NZ_CP027338:1089065-1089481 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB