Detailed information of TA system TA098063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 106040..106541
• Replicon: plasmid unnamed3
• Accession: NZ_CP027334
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A6X6XTU7
• Locus tag: MJ63_RS30225
• Protein ID: WP_000702245.1
• Coordinates: 106281..106541 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: MJ63_RS30220
• Protein ID: WP_001132407.1
• Coordinates: 106040..106291 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS30175	101691..102904	+	1214	WP_165963876.1	IS3 family transposase	-
MJ63_RS30185	103035..103472	-	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
MJ63_RS30190	103497..103679	-	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
MJ63_RS30195	104237..104419	+	183	WP_001481829.1	hypothetical protein	-
MJ63_RS30200	104526..104792	-	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
MJ63_RS30205	105212..105490	-	279	WP_000545937.1	hypothetical protein	-
MJ63_RS30220	106040..106291	-	252	WP_001132407.1	transcriptional regulator	Antitoxin
MJ63_RS30225	106281..106541	-	261	WP_000702245.1	hypothetical protein	Toxin
MJ63_RS30230	106593..106817	-	225	WP_000814381.1	hypothetical protein	-
MJ63_RS30235	106914..107123	-	210	WP_000866036.1	hypothetical protein	-
MJ63_RS30240	107164..107487	-	324	WP_000427403.1	hypothetical protein	-
MJ63_RS30250	108017..108391	-	375	WP_021500037.1	hypothetical protein	-
MJ63_RS31380	108442..108732	-	291	WP_170995147.1	hypothetical protein	-
MJ63_RS30260	108751..109116	+	366	WP_032345630.1	hypothetical protein	-
MJ63_RS30265	109116..110303	+	1188	WP_000937595.1	IS91 family transposase	-
MJ63_RS30275	110541..110945	+	405	WP_000839179.1	IS66 family insertion sequence hypothetical protein	-
MJ63_RS30280	110942..111289	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / hlyD / toxB / toxB / toxB / espP	1..181066	181066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9818.23 Da
• Isoelectric Point: 9.5163

>T98063 WP_000702245.1 NZ_CP027334:c106541-106281 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T98063 NZ_CP027334:c106541-106281 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT98063 WP_001132407.1 NZ_CP027334:c106291-106040 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT98063 NZ_CP027334:c106291-106040 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A6X6XTU7