Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 76050..76585 Replicon plasmid unnamed3
Accession NZ_CP027334
Organism Escherichia coli strain 2013C-3277

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag MJ63_RS30035 Protein ID WP_001270417.1
Coordinates 76298..76585 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag MJ63_RS30030 Protein ID WP_001132895.1
Coordinates 76050..76301 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ63_RS30000 71900..72265 - 366 WP_032345630.1 hypothetical protein -
MJ63_RS30010 72347..72949 + 603 Protein_84 DDE-type integrase/transposase/recombinase -
MJ63_RS30015 73170..74132 - 963 WP_032345409.1 ParB/RepB/Spo0J family partition protein -
MJ63_RS30020 74129..75334 - 1206 WP_000817635.1 AAA family ATPase -
MJ63_RS30030 76050..76301 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MJ63_RS30035 76298..76585 + 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MJ63_RS30040 76584..76832 + 249 Protein_89 integrase core domain-containing protein -
MJ63_RS30050 77512..78230 + 719 Protein_90 RepB family plasmid replication initiator protein -
MJ63_RS30055 78314..78964 + 651 WP_000993931.1 IS66 family insertion sequence hypothetical protein -
MJ63_RS30060 78964..79311 + 348 WP_000624618.1 IS66 family insertion sequence element accessory protein TnpB -
MJ63_RS30065 79331..80902 + 1572 WP_000381416.1 IS66 family transposase -
MJ63_RS30070 80931..81200 + 270 Protein_94 protein RepA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyC / hlyA / hlyB / hlyD / hlyD / toxB / toxB / toxB / espP 1..181066 181066
- inside IScluster/Tn - - 78314..90440 12126


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T98058 WP_001270417.1 NZ_CP027334:76298-76585 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T98058 NZ_CP027334:76298-76585 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGTGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT98058 WP_001132895.1 NZ_CP027334:76050-76301 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT98058 NZ_CP027334:76050-76301 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References