Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 36607..37250 Replicon plasmid unnamed3
Accession NZ_CP027334
Organism Escherichia coli strain 2013C-3277

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag MJ63_RS29840 Protein ID WP_001044768.1
Coordinates 36834..37250 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag MJ63_RS29835 Protein ID WP_001261287.1
Coordinates 36607..36837 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ63_RS29800 32480..33920 + 1441 Protein_44 enterohemolysin T1SS ABC transporter subunit EhxD -
MJ63_RS29805 33987..34082 + 96 Protein_45 transcriptional regulator -
MJ63_RS29810 34112..34273 + 162 Protein_46 lipid II-degrading bacteriocin -
MJ63_RS29815 34323..34677 - 355 Protein_47 colicin M immunity protein -
MJ63_RS29820 34849..35631 - 783 WP_001164190.1 site-specific integrase -
MJ63_RS29825 35633..36046 - 414 WP_000465041.1 hypothetical protein -
MJ63_RS29835 36607..36837 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MJ63_RS29840 36834..37250 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
MJ63_RS29845 37412..37912 - 501 WP_072279305.1 hypothetical protein -
MJ63_RS29850 37970..38820 + 851 Protein_53 IS3 family transposase -
MJ63_RS29855 39001..40458 - 1458 Protein_54 IS66-like element ISEc8 family transposase -
MJ63_RS29860 40508..41185 + 678 WP_100699708.1 IS66-like element accessory protein TnpA -
MJ63_RS29865 41185..41532 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyC / hlyA / hlyB / hlyD / hlyD / toxB / toxB / toxB / espP 1..181066 181066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T98057 WP_001044768.1 NZ_CP027334:36834-37250 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T98057 NZ_CP027334:36834-37250 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT98057 WP_001261287.1 NZ_CP027334:36607-36837 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT98057 NZ_CP027334:36607-36837 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAATCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References