Detailed information of TA system TA098056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 15631..16178
• Replicon: plasmid unnamed3
• Accession: NZ_CP027334
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: MJ63_RS29680
• Protein ID: WP_000969996.1
• Coordinates: 15631..15912 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: MJ63_RS29685
• Protein ID: WP_032209716.1
• Coordinates: 15909..16178 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS29650	10735..11922	+	1188	WP_000937595.1	IS91 family transposase	-
MJ63_RS29655	12199..12564	+	366	WP_032345630.1	hypothetical protein	-
MJ63_RS29660	12564..13751	+	1188	WP_000937595.1	IS91 family transposase	-
MJ63_RS29665	13937..14053	+	117	Protein_22	transposase domain-containing protein	-
MJ63_RS29670	14207..14794	-	588	WP_032345404.1	serine acetyltransferase	-
MJ63_RS29675	15094..15435	+	342	WP_000578011.1	hypothetical protein	-
MJ63_RS29680	15631..15912	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MJ63_RS29685	15909..16178	-	270	WP_032209716.1	hypothetical protein	Antitoxin
MJ63_RS29700	17091..17948	-	858	WP_032345402.1	incFII family plasmid replication initiator RepA	-
MJ63_RS29705	17941..18015	-	75	WP_001370046.1	RepA leader peptide Tap	-
MJ63_RS29715	18251..18505	-	255	WP_000083830.1	replication regulatory protein RepA	-
MJ63_RS29720	18746..19336	-	591	WP_000766812.1	DUF2726 domain-containing protein	-
MJ63_RS29725	19374..19583	-	210	WP_001302200.1	hemolysin expression modulator Hha	-
MJ63_RS29730	19629..20090	-	462	WP_001233850.1	thermonuclease family protein	-
MJ63_RS29740	20334..20546	-	213	WP_032345400.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyC / hlyA / hlyB / hlyD / hlyD / toxB / toxB / toxB / espP	1..181066	181066

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T98056 WP_000969996.1 NZ_CP027334:c15912-15631 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T98056 NZ_CP027334:c15912-15631 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10208.57 Da
• Isoelectric Point: 8.8735

>AT98056 WP_032209716.1 NZ_CP027334:c16178-15909 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQIAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT98056 NZ_CP027334:c16178-15909 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCAGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGATTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3