Detailed information of TA system TA098051

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 3602..4032
• Replicon: plasmid unnamed1
• Accession: NZ_CP027332
• Organism: Escherichia coli strain 2013C-3277

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: MJ63_RS31085
• Protein ID: WP_001388374.1
• Coordinates: 3602..3766 (-)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6Y0EDY2
• Locus tag: MJ63_RS29160
• Protein ID: WP_000079939.1
• Coordinates: 3763..4032 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MJ63_RS29130	1..350	+	350	WP_106913261.1	phosphotransferase	-
MJ63_RS29135	350..1186	+	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
MJ63_RS29140	1293..1697	-	405	Protein_2	IS91 family transposase	-
MJ63_RS29145	1809..2189	-	381	WP_001696636.1	HTH domain-containing protein	-
MJ63_RS29155	2631..3554	-	924	WP_001415483.1	hypothetical protein	-
MJ63_RS31085	3602..3766	-	165	WP_001388374.1	hypothetical protein	Toxin
MJ63_RS29160	3763..4032	-	270	WP_000079939.1	hypothetical protein	Antitoxin
MJ63_RS31340	4105..4245	-	141	WP_001404367.1	hypothetical protein	-
MJ63_RS29175	4959..5981	-	1023	WP_032268691.1	replication initiation protein	-
MJ63_RS29180	5974..6048	-	75	WP_071703955.1	RepA leader peptide Tap	-
MJ63_RS29190	6284..6541	-	258	WP_047648528.1	replication regulatory protein RepA	-
MJ63_RS29200	7098..7328	-	231	WP_170867393.1	hypothetical protein	-
MJ63_RS29205	7661..8674	-	1014	WP_001404372.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id	-	1..20839	20839

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6604.73 Da
• Isoelectric Point: 7.1989

>T98051 WP_001388374.1 NZ_CP027332:c3766-3602 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T98051 NZ_CP027332:c3766-3602 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAATACATTGCGGAGCAGAACCTGAT
AGCAGCCATTGAACTGGACGAACGGATTGGTTATTTCGGACGAACGCACCGGCTTACGCCGGTTTTACATCTGTACTACA
TGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 6.7263

>AT98051 WP_000079939.1 NZ_CP027332:c4032-3763 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT98051 NZ_CP027332:c4032-3763 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCACGGCGATGTACTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGAGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6Y0EDY2