Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 5222053..5222671 Replicon chromosome
Accession NZ_CP027331
Organism Escherichia coli strain 2013C-3277

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MJ63_RS27965 Protein ID WP_001291435.1
Coordinates 5222053..5222271 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MJ63_RS27970 Protein ID WP_000344800.1
Coordinates 5222297..5222671 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ63_RS27930 5217342..5217914 + 573 WP_000779826.1 lipoprotein -
MJ63_RS27935 5217945..5218256 - 312 WP_000409911.1 MGMT family protein -
MJ63_RS27945 5218635..5218988 + 354 WP_000878140.1 DUF1428 domain-containing protein -
MJ63_RS27950 5219030..5220580 - 1551 WP_001342068.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MJ63_RS27955 5220744..5221214 - 471 WP_000136192.1 YlaC family protein -
MJ63_RS27960 5221330..5221881 - 552 WP_000102564.1 maltose O-acetyltransferase -
MJ63_RS27965 5222053..5222271 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
MJ63_RS27970 5222297..5222671 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MJ63_RS27975 5223217..5226366 - 3150 WP_001132475.1 multidrug efflux RND transporter permease subunit -
MJ63_RS27980 5226389..5227582 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T98050 WP_001291435.1 NZ_CP027331:c5222271-5222053 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T98050 NZ_CP027331:c5222271-5222053 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT98050 WP_000344800.1 NZ_CP027331:c5222671-5222297 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT98050 NZ_CP027331:c5222671-5222297 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References