Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 1570556..1570988 Replicon chromosome
Accession NZ_CP027331
Organism Escherichia coli strain 2013C-3277

Toxin (Protein)


Gene name parE_1 Uniprot ID -
Locus tag MJ63_RS08895 Protein ID WP_050439653.1
Coordinates 1570749..1570988 (+) Length 80 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag MJ63_RS08890 Protein ID WP_001302048.1
Coordinates 1570556..1570747 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ63_RS08840 1565634..1565852 - 219 WP_000683609.1 DUF4014 family protein -
MJ63_RS08845 1565980..1566291 - 312 WP_001289673.1 hypothetical protein -
MJ63_RS08850 1566284..1566511 - 228 WP_000699809.1 hypothetical protein -
MJ63_RS08855 1566508..1566789 - 282 WP_000603384.1 DNA-binding protein -
MJ63_RS08860 1566822..1567583 - 762 WP_000450872.1 DUF1627 domain-containing protein -
MJ63_RS08865 1567609..1568350 - 742 Protein_1624 ATP-binding protein -
MJ63_RS08870 1568357..1569319 - 963 WP_001444941.1 helix-turn-helix domain-containing protein -
MJ63_RS08875 1569342..1569767 - 426 WP_000693943.1 Rha family transcriptional regulator -
MJ63_RS08880 1569764..1570066 - 303 WP_000172738.1 transcriptional regulator -
MJ63_RS08885 1570164..1570535 + 372 WP_001169687.1 hypothetical protein -
MJ63_RS08890 1570556..1570747 + 192 WP_001302048.1 hypothetical protein Antitoxin
MJ63_RS08895 1570749..1570988 + 240 WP_050439653.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MJ63_RS08900 1571314..1571466 + 153 WP_001427316.1 DUF1391 family protein -
MJ63_RS08905 1571887..1572108 + 222 WP_000560218.1 killing protein KilR -
MJ63_RS08910 1572102..1572278 + 177 WP_000245534.1 hypothetical protein -
MJ63_RS08915 1572352..1572627 + 276 WP_001307773.1 hypothetical protein -
MJ63_RS08920 1572726..1575377 + 2652 WP_032346644.1 exodeoxyribonuclease VIII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espFu/tccP 1531673..1583908 52235


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-75)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 80 a.a.        Molecular weight: 9114.45 Da        Isoelectric Point: 4.6470

>T98025 WP_050439653.1 NZ_CP027331:1570749-1570988 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSV

Download         Length: 240 bp

>T98025 NZ_CP027331:1570749-1570988 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT98025 WP_001302048.1 NZ_CP027331:1570556-1570747 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT98025 NZ_CP027331:1570556-1570747 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References