Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 22712..23259 Replicon plasmid unnamed2
Accession NZ_CP027327
Organism Escherichia coli strain 2013C-4830

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag C6N65_RS27960 Protein ID WP_000970007.1
Coordinates 22712..22993 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag C6N65_RS27965 Protein ID WP_000079941.1
Coordinates 22990..23259 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N65_RS27930 19339..19941 + 603 WP_000517695.1 hypothetical protein -
C6N65_RS27935 19997..20491 + 495 WP_001459505.1 hypothetical protein -
C6N65_RS28980 20491..20757 + 267 WP_000955263.1 hypothetical protein -
C6N65_RS27950 21234..21707 - 474 WP_001334658.1 hypothetical protein -
C6N65_RS27955 21824..22672 - 849 WP_001057996.1 3'-5' exonuclease -
C6N65_RS27960 22712..22993 - 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6N65_RS27965 22990..23259 - 270 WP_000079941.1 hypothetical protein Antitoxin
C6N65_RS28985 23314..23472 - 159 WP_000841008.1 hypothetical protein -
C6N65_RS27980 24168..25199 - 1032 WP_000907863.1 replication initiation protein -
C6N65_RS27995 26172..26435 + 264 WP_001324596.1 ash family protein -
C6N65_RS28000 26353..26640 + 288 WP_000074849.1 conjugal transfer protein TraA -
C6N65_RS28010 27082..27615 + 534 WP_001306770.1 transcription termination factor NusG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..93170 93170


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T98011 WP_000970007.1 NZ_CP027327:c22993-22712 [Escherichia coli]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T98011 NZ_CP027327:c22993-22712 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT98011 WP_000079941.1 NZ_CP027327:c23259-22990 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT98011 NZ_CP027327:c23259-22990 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References