98010

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9103..9628 Replicon plasmid unnamed2
Accession NZ_CP027327
Organism Escherichia coli strain 2013C-4830

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag C6N65_RS27865 Protein ID WP_001159868.1
Coordinates 9103..9408 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C6N65_RS27870 Protein ID WP_000813634.1
Coordinates 9410..9628 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6N65_RS27840 5274..5690 - 417 WP_001278818.1 plasmid partitioning/stability family protein -
C6N65_RS27845 5683..6663 - 981 WP_000688514.1 plasmid segregation protein ParM -
C6N65_RS27850 7077..7385 - 309 WP_000030199.1 hypothetical protein -
C6N65_RS27855 7472..8116 - 645 WP_001144036.1 ParA family protein -
C6N65_RS27860 8296..9102 - 807 WP_106918818.1 site-specific integrase -
C6N65_RS27865 9103..9408 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
C6N65_RS27870 9410..9628 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6N65_RS27875 10188..11114 + 927 WP_001290416.1 hypothetical protein -
C6N65_RS27880 11159..11413 + 255 WP_000678527.1 hypothetical protein -
C6N65_RS27885 11912..12256 + 345 WP_000793307.1 hypothetical protein -
C6N65_RS27890 12432..13433 - 1002 WP_106918819.1 DUF4238 domain-containing protein -
C6N65_RS27895 13784..14041 + 258 WP_000371888.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..93170 93170


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T98010 WP_001159868.1 NZ_CP027327:c9408-9103 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T98010 NZ_CP027327:c9408-9103 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT98010 WP_000813634.1 NZ_CP027327:c9628-9410 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT98010 NZ_CP027327:c9628-9410 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References