Detailed information of TA system TA098008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 37417..37952
• Replicon: plasmid unnamed1
• Accession: NZ_CP027326
• Organism: Escherichia coli strain 2013C-4830

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C6N65_RS27560
• Protein ID: WP_001270421.1
• Coordinates: 37417..37704 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: C6N65_RS27565
• Protein ID: WP_001132895.1
• Coordinates: 37701..37952 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6N65_RS27530	33144..33964	-	821	Protein_31	transposase	-
C6N65_RS27535	34059..34279	+	221	Protein_32	serine/threonine protein kinase	-
C6N65_RS27540	34400..35140	+	741	WP_032208234.1	site-specific integrase	-
C6N65_RS27545	35383..36360	-	978	WP_032208235.1	RepB family plasmid replication initiator protein	-
C6N65_RS27555	37028..37415	-	388	Protein_35	DDE-type integrase/transposase/recombinase	-
C6N65_RS27560	37417..37704	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6N65_RS27565	37701..37952	-	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6N65_RS27570	38126..38311	-	186	WP_032208250.1	hypothetical protein	-
C6N65_RS27575	38476..39653	-	1178	Protein_39	IS91 family transposase	-
C6N65_RS27580	39604..40017	-	414	WP_001344870.1	hypothetical protein	-
C6N65_RS27585	40106..40296	-	191	Protein_41	IS3 family transposase	-
C6N65_RS27590	40531..42741	+	2211	WP_000592771.1	catalase/peroxidase KatP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyD / hlyB / hlyA / hlyC / papC / espP	1..74671	74671
	-	inside	IScluster/Tn	-	-	31951..44070	12119

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T98008 WP_001270421.1 NZ_CP027326:c37704-37417 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T98008 NZ_CP027326:c37704-37417 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCAGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT98008 WP_001132895.1 NZ_CP027326:c37952-37701 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT98008 NZ_CP027326:c37952-37701 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTGGACTTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6